LOCUS       sequence-111936-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of 6xHis-(-30)GFP in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-6xHis-(-30)GFP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat ataccatggg tcatcaccac caccatcacg gtggcgctag
      181 caaaggtgaa gagctgtttg acggtgtagt accgatctta gtggaattag acggcgacgt
      241 gaacggtcac gaatttagcg tgcgcggcga gggcgaaggt gacgctaccg agggtgaatt
      301 gaccctgaag tttatttgca caacaggcga attacccgtt ccgtggccca ccttagtgac
      361 caccctgacc tatggcgttc agtgcttcag tgattaccca gatcatatgg atcaacacga
      421 ttttttcaaa tcagccatgc ctgaaggata tgttcaagag cgtacaatca gcttcaagga
      481 cgatggcacc tataaaacgc gtgcggaagt gaaatttgaa ggcgacacat tagtaaaccg
      541 tatcgaactg aaaggtatcg acttcaaaga agacggcaac attttaggcc ataagctgga
      601 atataacttt aattctcatg acgtgtatat tacggccgat aaacaggaaa acggtatcaa
      661 ggcagaattt gaaattcgcc ataacgtgga ggacggcagc gttcaattag cggatcatta
      721 tcaacaaaac acgccgattg gtgatgggcc tgtactgtta cctgacgatc actacctgag
      781 cacggagtca gccctgagca aagatccgaa cgaagaccgc gatcacatgg ttctgttaga
      841 attcgtgacc gctgcaggca ttgatcatgg aatggacgag ctgtac
//
