LOCUS       sequence-111938-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of 6xHis-(+36)GFP in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-6xHis-(pos36)GFP
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatgggtc atcaccacca ccatcacggt ggcgctagca
      181 aaggtgaacg tctgtttcgt ggtaaagtac cgatcttagt ggaattaaag ggcgacgtga
      241 acggtcataa atttagcgtg cgcggcaaag gcaaaggtga cgctacccgt ggtaaattga
      301 ccctgaagtt tatttgcaca acaggcaaat tacccgttcc gtggcccacc ttagtgacca
      361 ccctgaccta tggcgttcag tgcttcagtc gttaccctaa acatatgaaa cgtcacgatt
      421 ttttcaaatc agccatgcct aaaggatatg ttcaagagcg tacaatcagc ttcaagaagg
      481 atggcaaata taaaacgcgt gcggaagtga aatttgaagg ccgcacatta gtaaatcgta
      541 tcaaactgaa aggtcgtgac ttcaaagaaa aaggcaacat tttaggccat aaactgcgtt
      601 ataactttaa ttctcataag gtgtatatta cggccgataa acgcaagaat ggtatcaagg
      661 caaaattcaa aattcgccat aacgtgaaag acggcagcgt tcaattagcg gatcattatc
      721 aacaaaacac gccgattggt cgcgggcctg tactgttacc tcgcaaccac tacctgagca
      781 cccgttctaa actgagcaaa gatccgaaag aaaaacgcga tcacatggtt ctgttagaat
      841 tcgtgaccgc tgcaggcatt aagcacggac gcgacgaacg ctacaagtaa taatgaggta
      901 ccctcgagtc tggtaa
//
