LOCUS       sequence-111943-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pREV1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK027
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(858..892)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc aaacggtgtt gttatccgat acaaccggat atttttcttt taatgagtct
      181 aaaccgtgat agcttcaggt taatacaatc aaaaaaagct caaatattct tttaatgccg
      241 cgttcacaga ttccaattga atacaactag gtagttcatt atatgaagcc tttgctacta
      301 tttttcacta tagtctgcct tcaccttaat gcagacatcc acatatttta atcactttaa
      361 aataaaaagg aagatatatt agaagctatg atccaatctg taagccagat taaaattcac
      421 gaactcttct ttcatttgaa ttgaatgctt tgagttgggg tagattatcg caaattactc
      481 atcacattta ttgactacga acttgctgat gtcctttttt tatttatatt tttcttcagt
      541 gaagcgattt tttttttaca cagaccaaga cggaaaaaag tagctaagga agaaaacaaa
      601 atcatgaaaa aaatgtgaag tgatcatgca catcgcatca acttaaacat tggcttagag
      661 atatatagag ttagagttta cggcaacctt taagcaccaa taccttttgg catagtctaa
      721 agacctggtt cttaatttta aacaaattta actaaagatt tccctatcaa agaagtaacg
      781 agttgacaga ttttctcaaa ataaatcgat actgcatttc taggcatatc cagcgagatc
      841 tatgtgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
      901 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
      961 aattacgccc cgccctgcca ctc
//
