LOCUS       sequence-111947-        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate 
            Dependent E. coli Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSMART-Mev1
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..83
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      99..126
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      4552..4583
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        4584..4686
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4687..5502
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4760..4779)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5526..5553
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(5565..6152)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5645..5664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6174..6203)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      121 tctgcgttta tacacagcta acaccacgtc gtccctatct gctgccctag gtctatgagt
      181 ggttgctgga taacgtgcgt aattgtgctg atctcttata tagctgctct cattatctct
      241 ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt
      301 aatacaaagc ctgtaaaacg tcaggataac ttctatattc agggagacca caacggtttc
      361 cctctacaaa taattttgtt taactttcgt ggaaaaagga gatataccat gaagacggta
      421 gttattatcg acgcactgcg tacccccatt ggaaaataca aaggaagtct gagccaggta
      481 agcgccgtcg acctgggcac acatgtgacc acgcagttgt tgaagcgtca cagcactatc
      541 agcgaggaaa ttgatcaggt catttttggt aatgttctgc aggcgggcaa tgggcagaac
      601 cctgcacgtc agattgcaat caactcaggt ttaagccatg aaattccagc gatgacggtc
      661 aatgaggtct gtggcagtgg gatgaaagcg gtaatcctgg ccaaacagtt aatccagctg
      721 ggtgaggcgg aggtacttat cgcaggtggt attgaaaaca tgtcacaggc cccgaaactg
      781 caacgcttta actacgaaac agaaagctac gatgcgcctt tttcgtccat gatgtatgat
      841 ggtcttaccg acgcattcag tggtcaggcg atgggtctga cggccgagaa tgttgctgaa
      901 aaataccacg ttacccgtga ggaacaagac caattctctg tccatagcca actcaaagcg
      961 gcacaggctc aggcagaagg catttttgcc gatgagattg caccactgga agtttccggc
     1021 accctggtgg aaaaggacga gggcattcgt ccgaatagca gtgttgaaaa actcggtact
     1081 ttgaaaaccg tattcaaaga ggacggcacg gtgactgccg gtaatgcctc aactatcaac
     1141 gacggtgcct cggcactgat tattgcgtct caagaatacg cggaagcgca cggcttgccg
     1201 tatctcgcga ttatccgcga ttcagtggag gtcggcatcg atcccgcgta catgggcatt
     1261 tcgccgatca aagcaattca gaagcttctg gcacgcaacc agttgacgac cgaagagatt
     1321 gatttatacg aaatcaatga agcgttcgcg gcgacctcga ttgtggttca gcgtgaactt
     1381 gccctcccgg aagaaaaggt caacatctat ggcggaggca tcagtttggg ccatgccatc
     1441 ggagcgaccg gtgcccgtct gctcaccagc ttatcatatc agttgaacca gaaagaaaaa
     1501 aagtacggcg ttgcatctct gtgtattggc ggaggtctgg gcctcgccat gttgttagaa
     1561 cgtccgcagc aaaaaaaaaa ctcccgcttt tatcagatgt cgccggagga acgtctggcg
     1621 agcttgttga acgaagggca gatctctgcc gacactaaaa aggaattcga aaacacggca
     1681 ctgagcagtc agattgcgaa ccatatgatt gaaaatcaga tcagcgagac cgaggtgccc
     1741 atgggcgtgg gccttcatct cacggtggac gaaacggatt atctggtacc aatggccaca
     1801 gaagaaccgt cggtaatcgc cgcgttgtca aatggcgcga aaatcgcgca agggttcaaa
     1861 acggtcaacc agcagcgtct catgcgcggc cagatcgtgt tctatgatgt agcagatgca
     1921 gagagtctga ttgacgagtt acaggttcgt gagacggaga tttttcagca agccgagctg
     1981 tcgtacccga gcattgttaa acgtggcggt ggccttcgtg acttgcagta tcgcgccttc
     2041 gacgaatcgt tcgtgagtgt cgactttctg gtagacgtga aggacgccat gggggccaat
     2101 atcgttaatg ccatgctgga aggggttgca gagctgtttc gtgagtggtt cgccgaacaa
     2161 aaaatcctgt ttagcatctt aagcaattac gcaacggaaa gcgtcgtgac catgaaaacc
     2221 gcgatccctg ttagccgcct ttcaaagggc agtaacggtc gtgaaatcgc tgaaaaaatt
     2281 gttctcgcgt cccgctatgc atcgttggat ccttatcgcg cggtgacaca caacaaaggc
     2341 attatgaatg gtatcgaagc ggtcgttctg gcgaccggca acgatactcg cgccgtgagc
     2401 gcgtcctgcc atgcttttgc tgtgaaagag ggccgttatc agggcttgac gtcctggacc
     2461 ctggacggtg aacagctgat cggcgaaatc tcggtgcccc tcgccctggc cactgtgggc
     2521 ggcgccacaa aagtgttgcc aaaaagccaa gcggcggcgg atctgctggc cgtaactgat
     2581 gctaaggaac tgagtcgcgt ggttgccgca gtgggcctgg cccaaaacct ggcagcactg
     2641 cgcgcgctgg tttctgaagg catccagaaa ggtcatatgg ccctgcaagc gcgctctctg
     2701 gccatgaccg taggggcgac cggcaaggaa gtcgaagcgg tagctcaaca gttaaaacgc
     2761 cagaaaacta tgaatcagga tcgtgcgctg gccatcctca atgacctgcg caaacagtaa
     2821 tagtcgcgcc gaaaaccccg cttcggcggg gttttgccgc acgtctccat cgcttgccca
     2881 agttgtgaag cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
     2941 ttgctggata accatccata aattttgcat aattaatgta aagaccaggc tcgccagtaa
     3001 cgctaaattc atttggctgt aagcgcggtg tcatccgcgt caggaaaatt aaacagttac
     3061 tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg
     3121 cccgctaaac atttgtttat attcagggag accacaacgg tttccctcta caaataattt
     3181 tgtttaactt tgctggaaaa aggagatata ccatgaccat tgggattgat aaaatctcgt
     3241 ttttcgtgcc tccttattat atcgacatga cggccctggc cgaggctcgc aatgtggatc
     3301 ccggcaaatt tcacatcggt atcggccagg accaaatggc ggtgaatccc atctcgcagg
     3361 acattgtcac cttcgccgca aacgcagcag aagctatctt gactaaagaa gataaagagg
     3421 ccatcgacat ggtgatcgtg ggtacggaaa gctctattga cgaaagtaaa gccgcggcgg
     3481 tggtattaca ccgcctgatg ggtatccagc cgtttgcgcg ctcctttgaa atcaaagagg
     3541 cctgctacgg cgcaacggct ggactgcaac tcgcgaagaa ccatgttgca ttacatccgg
     3601 ataaaaaagt cctggttgtc gcggcggaca tcgcgaaata cggcctgaac tccggcgggg
     3661 aaccaacgca gggtgccggc gcagtggcga tgcttgtcgc aagcgagcct cgtatcctgg
     3721 ctttaaagga ggacaacgtg atgctgacac aggatattta cgatttttgg cgtcccaccg
     3781 gtcatccata tccgatggtt gatggtcctc tgtccaatga aacttatatt cagagcttcg
     3841 cgcaagtttg ggatgaacat aagaaacgta ccggtctgga ttttgcggat tacgacgctc
     3901 tggcttttca cattccatac acgaaaatgg gcaaaaaagc cctcttagct aaaatctcag
     3961 accagaccga ggcagaacag gaacgcattt tagcgcgtta cgaagagtca attatctaca
     4021 gccgccgtgt aggtaattta tatacggggt cgctttatct gggattgatt tccttactcg
     4081 aaaacgccac aaccctgacg gcgggtaacc aaatcggttt attctcttac ggtagcggtg
     4141 ccgttgccga attcttcacg ggtgagctgg ttgccggtta ccagaaccac ttacagaaag
     4201 aaacccacct cgccctgctg gacaaccgta ctgaactcag catcgcagaa tatgaggcca
     4261 tgttcgccga aacactcgac acggatatcg atcaaacctt agaggatgaa ctcaaatatt
     4321 ccatttcagc gattaataac accgtccgct cctatcgcaa ttagtaagat cgtcccggct
     4381 tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga
     4441 ggggcagaaa gatgaatgac tgtccacgac gctataccca aaagaaagac gaattctcta
     4501 gatatcgctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
     4561 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
     4621 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     4681 gctaaaatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     4741 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     4801 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     4861 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt
     4921 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     4981 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     5041 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     5101 tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     5161 ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     5221 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     5281 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     5341 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     5401 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     5461 aataaattgc agtttcactt gatgctcgat gagtttttct aatgagggcc caaatgtaat
     5521 cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca taggctccgc
     5581 ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga
     5641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga aaaagagttg
     6061 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6121 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc taccgaagaa
     6181 aggcccaccc gtgaaggtga gccagtgagt tgattgcagt ccagttacgc tggagtctga
     6241 ggctcgtcct gaatgatatc aagcttgaat tcgtt
//
