LOCUS       sequence-111949-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pMF_2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK029
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(907..941)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca aacgttgatc tgccttttat agctaagaaa aaaaaagaga aaaaaataat
      181 aagtgcgggt aacctcatgc attgtaaaac ggtagggcta caaggcacta cttactctac
      241 gctatagaag aagcttcaag acatggtgtt gttcttattt gagcgaactc agctgtcacc
      301 gtttcgggaa actctatagt tttctgcgtt tcagtacgca gttgggcgtg ctaaagttgt
      361 tttcctaatt tgctagtgtt cacttgctca ttgatgtccc taaaaggtaa tgtcgcatag
      421 taagatgtat gtcccatatc aaacgcattc ctagaaaaac atgcactgaa tgaataagca
      481 gttacttcga ttactaatct tcacagcata atttttctct tatcttgtaa acctctttct
      541 atcttttcat aaagtttgtc acttatattt cttcattggt acatcaatgc cagcaacgat
      601 gtgcgcatct gggcgacgcc tgtagtgatt gttttcaagg tatcgagcca aactattcat
      661 cgttactgtt tcaaatattc agttgtttca gtacagagtc gccgtggacc tagtgaaact
      721 tggtgtcttt acagcgcaga gatgagggct tatatgtata aaagctgtcc ttgattctgg
      781 tgtagtttga ggtgtccttc ctatatctgt ttttatattc tatataatgg ataattacta
      841 ccatcacctg catcaaattc cagtaaattc acatattgga gaaaagatct atgtgagacc
      901 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
      961 gaagg
//
