LOCUS       sequence-111952-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK030
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        216..657
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    216..333
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     628..651
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      complement(691..725)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc aaacgcccca ttatcttagc ctaaaaaaac cttctctttg gaactttcag
      181 taatacgctt aactgctcat tgctatattg aagtacggat tagaagccgc cgagcgggcg
      241 acagccctcc gacggatgac tctcctccgt gcgtcctcgt catcaccggt cgcgttcctg
      301 aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac aatactagct
      361 tttatggtta tgaagaggaa aaattggcag taacctggcc ccacaaacct tcaaattaac
      421 gaatcaaatt aacaaccata ggatgataat gcgattagtt ttttagcctt atttctgggg
      481 taattaatca gcgaagcgat gatttttgat ctattaacag atatataaat ggaaaagctg
      541 cataaccact ttaactaata ctttcaacat tttcagtttg tattacttct tattcaaatg
      601 tcataaaagt atcaacaaaa aattgttaat atacctctat actttaacgt caaggagaaa
      661 aaactataag atctatgtga gaccagacca ataaaaaacg cccggcggca accgagcgtt
      721 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      781 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      841 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      901 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      961 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1021 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggttt
//
