LOCUS       sequence-111955-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(S219-G521R) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD111e - intein-Cas9(S219-G521R)
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc
       61 cgccaccatg gataaaaagt attctattgg tttagacatc ggcactaatt ccgttggatg
      121 ggctgtcata accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac
      181 agaccgtcat tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac
      241 ggcagaggcg actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg
      301 aatatgttac ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt
      361 tcaccgtttg gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat
      421 ctttggaaac atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct
      481 cagaaaaaag ctagttgact caactgataa agcggacctg aggttaatct acttggctct
      541 tgcccatatg ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa
      601 ctcggatgtc gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga
      661 gaaccctata aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa
      721 atgccttgcc gagggtaccc gaatcttcga tccggtcact ggtacaacgc atcgcatcga
      781 ggatgttgtc gatgggcgca agcctattca tgtcgtggct gctgccaagg acggaacgct
      841 gctcgcgcgg cccgtggtgt cctggttcga ccagggaacg cgg
//
