LOCUS       sequence-111958-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK033
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..846
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      complement(866..900)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc atatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
      181 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
      241 gctacttacg gtaaattgac cttaaaattg atttgtacta ctggtaaatt gccagttcca
      301 tggccaacct tagtcactac tttaggttat ggtttgcaat gttttgctag atacccagat
      361 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
      421 actatttttt tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt
      481 gataccttag ttaatagaat cgaattaaaa ggtattgatt ttaaagaagg tggtaacatt
      541 ttaggtcaca aattggaata caactataac tctcacaatg tttacatcac tgctgacaaa
      601 caaaagaatg gtatcaaagc taacttcaaa attagacaca acattgaaga tggtggtgtt
      661 caattagctg accattatca acaaaatact ccaattggtg atggtccagt cttgttacca
      721 gacaaccatt acttatccta tcaatctgcc ttatccaaag atccaaacga aaagagagat
      781 cacatggtct tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg
      841 tacaaaggat cctgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
      901 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
      961 cgatatcaaa ttacgccccg ccctgccact catcgca
//
