LOCUS       sequence-111960-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK034
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             132..842
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      complement(862..896)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca tatggtgtcc aaaggagagg agttaatcaa ggaaaacatg agaatgaaag
      181 ttgtcatgga gggctccgtt aatggtcacc aattcaagtg tacaggggaa ggtgaaggta
      241 atccttacat gggtacacaa actatgagaa ttaaagtaat tgaaggcgga ccactaccat
      301 ttgcatttga cattctggca acgtcattca tgtacggatc acgaactttc atcaagtacc
      361 ctaaaggtat accagacttt ttcaagcaat cttttccaga gggttttaca tgggaaaggg
      421 ttacaagata cgaagatggg ggtgtcgtca cagttatgca agatacttca ttagaagatg
      481 gctgccttgt ctatcatgtg caagtaagag gggtgaattt tccttctaac ggacctgtga
      541 tgcagaaaaa gaccaaaggt tgggaaccaa atactgaaat gatgtaccca gctgatggag
      601 gtttgagagg ctacacacac atggcgctta aagttgatgg tggaggtcat ttgtcttgta
      661 gttttgttac cacttatcgt tctaaaaaga ctgttggcaa tatcaaaatg ccaggaatac
      721 atgctgtaga ccacagacta gaaagactcg aagagagcga taacgaaatg ttcgttgtac
      781 agagagagca tgccgtagcc aaatttgctg gcttaggcgg tggtatggat gaattgtata
      841 agggatcctg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      901 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      961 atcaaattac gccccgccct gccactc
//
