LOCUS       sequence-111962-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK035
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(859..893)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca tatgaagaac atcaagaaga accaagttat gaacttgggt ccaaactcta
      181 agttgttgaa ggaatacaag tctcaattga tcgaattgaa catcgaacaa ttcgaagctg
      241 gtatcggttt gatcttgggt gacgcttaca tccgatctcg cgacgaaggt aagacctact
      301 gtatgcaatt cgaatggaag aacaaggctt acatggacca cgtttgtttg ttgtacgacc
      361 aatgggtttt gtctccacca cacaagaagg aaagagttaa ccacttgggt aacttggtta
      421 tcacctgggg tgctcaaacc ttcaagcacc aagctttcaa caagttggct aacttgttca
      481 tcgttaacaa caagaaaacc atcccaaaca acttggttga aaactacttg accccaatgt
      541 ctttggctta ctggttcatg gacgacggtg gtaagtggga ctacaacaag aactctacca
      601 acaagtctat cgttttgaac acccaatctt tcaccttcga agaagttgaa tacttggtta
      661 agggtttgag aaacaagttc caattgaact gttacgttaa gatcaacaag aacaagccaa
      721 tcatctacat cgactctatg tcttacttga tcttctacaa cttgatcaag ccatacttga
      781 tcccacaaat gatgtacaag ttgccaaaca ccatctcttc tgaaaccttc ttgaagtaag
      841 gatcctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      901 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      961 aaattacgcc ccgccctgcc actcatc
//
