LOCUS       sequence-111963-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-(-30)GFP-9xGGS-Cre-6xHis
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gtcattagtg atggtggtgg tgatgggatc cgccgtcgcc atcttccagc aggcgcacca
      121 ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt acattggtcc
      181 agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata tctcgcgcgg
      241 ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag tcatccttag
      301 cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga gttgatagct
      361 ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac aggtagttat
      421 tcggatcatc agctacacca gagacggaaa tccatcgctc gaccagttta gttaccccca
      481 ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg ccaatatgga
      541 ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg gcaatttcgg
      601 ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt atatcctggc
      661 agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt tcgaacgcta
      721 gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatacgc cgcataacca
      781 gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga agcatgttta
      841 gctggcccaa atgttgctgg atagttt
//
