LOCUS       sequence-111965-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLUK
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..40)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     140..159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        423..662
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
       61 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcggacgt
      121 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca accataattc
      181 aaaatgacac cgattattta aagctgcagc atacgatata tatacatgtg tatatatgta
      241 tacctatgaa tgtcagtaag tatgtatacg aacagtatga tactgaagat gacaaggtaa
      301 tgcatcattc tatacgtgtc attctgaacg aggcgcgctt tccttttttc tttttgcttt
      361 ttcttttttt ttctcttgaa ctcgacgcgt cgaggaaaac tcttcaatag ttttgccagc
      421 ggaattccac ttgcaattac ataaaaaatt ccggcggttt ttcgcgtgtg actcaatgtc
      481 gaaatacctg cctaatgaac atgaacatcg cccaaatgta tttgaagacc cgctgggaga
      541 agttcaagat atataagtaa caagcagcca atagtataaa aaaaaatctg agtttattac
      601 ctttcctgga atttcagtga aaaactgcta attatagaga gatatcacag agttactcac
      661 taatgactaa cgaaaaggtc tggatagaga agttggataa tccaactctt tcagtgttac
      721 cacatgactt tttacgccca caacaagaac cttatacgaa acaagctaca tattcgttac
      781 agctacctca gctcgatgtg cctcatgata gtttttctaa caaatacgct gtcgctttga
      841 gtgtatgggc tgcattgata tatagagtaa ccggtgacga tgatattgtt ctttatattg
      901 cgaataacaa aatcttaaga ttcaatattc aaccaacgtg gtcatttaat gagctgtatt
      961 ctacaattaa caatgagttg aacaagctca attctattga ggccaatttt
//
