LOCUS       sequence-111974-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLUK
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     193..215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     207..224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     208..224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        231..249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    258..365
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(377..395)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(416..432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(416..432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(429..451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    440..456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(464..470,471..488,489..494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    509..530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(647..664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(898..917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      121 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      181 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
      241 cactataggg cgaattggag ctccaccgcg gtggcggccg ctctagaact agtgggtccc
      301 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctggag ggggggcccg
      361 gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
      421 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      481 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      541 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      601 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
//
