LOCUS       sequence-111977-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-Cas9-6xHis
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat tatcattagt gatggtggtg gtgatggtca
       61 cctcctagct gactcaaatc aatgcgtgtt tcataaagac cagtgatgga ttgatggata
      121 agagtggcat ctaaaacttc ttttgtagac gtatatcgtt tacgatcaat tgttgtatca
      181 aaatatttaa aagcagcggg agctccaaga ttcgtcaacg taaataaatg aataatattt
      241 tctgcttgtt cacgtattgg tttgtctcta tgtttgttat atgcactaag aactttatct
      301 aaattggcat ctgctaaaat aacacgctta gaaaattcac tgatttgctc aataatctca
      361 tctaaataat gcttatgctg ctccacaaac aattgttttt gttcgttatc ttctggacta
      421 cccttcaact tttcataatg actagctaaa tataaaaaat tcacatattt gcttggcaga
      481 gccagctcat ttcctttttg taattctccg gcactagcca gcatccgttt acgaccgttt
      541 tctaactcaa aaagactata tttaggtagt ttaatgatta agtctttttt aacttcctta
      601 tatcctttag cttctaaaaa gtcaatcgga tttttttcaa aggaacttct ttccataatt
      661 gtgatcccta gtaactcttt aacggatttt aacttcttcg atttcccttt ttccacctta
      721 gcaaccacta ggactgaata agctaccgtt ggactatcaa aaccaccata tttttttgga
      781 tcccagtctt ttttacgagc aataagcttg tccgaatttc tttt
//
