LOCUS       sequence-111982-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ubi-M as a Type 3a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK041
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             132..359
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      complement(499..533)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca tatgcagatt ttcgttaaaa ctttaactgg taaaacaatt actttagaag
      181 ttgagtcatc tgatactatc gataatgtta aatctaagat ccaggataag gaaggtattc
      241 ctccagatca acaaagattg attttcgcag gtaagcagtt ggaggacggt aggactttgt
      301 ctgattataa catacagaaa gaatctactt tgcacttagt cttaagatta aggggaggta
      361 tgcatggttc tggtgcctgg ttgttacctg tatcattggt taaaagaaag accacattgg
      421 caccaaatac tcaaactgcc tctccaagag ccttggcaga ctctttaatg cagagatcag
      481 gttcttgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      541 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      601 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      661 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      721 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      781 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
      841 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      901 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      961 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     1021 ctcaccgtct ttcattgcca tacgaaa
//
