LOCUS       sequence-111983-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Cas9-NLS-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-Cas9-NLS-6xHis
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..53)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacgagtcca tgtgctggcg ttcaaattat cattagtgat ggtggtggtg atgcttatcc
       61 atcaccttcc tcttcttctt ggggtcacct cctagctgac tcaaatcaat gcgtgtttca
      121 taaagaccag tgatggattg atggataaga gtggcatcta aaacttcttt tgtagacgta
      181 tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag cagcgggagc tccaagattc
      241 gtcaacgtaa ataaatgaat aatattttct gcttgttcac gtattggttt gtctctatgt
      301 ttgttatatg cactaagaac tttatctaaa ttggcatctg ctaaaataac acgcttagaa
      361 aattcactga tttgctcaat aatctcatct aaataatgct tatgctgctc cacaaacaat
      421 tgtttttgtt cgttatcttc tggactaccc ttcaactttt cataatgact agctaaatat
      481 aaaaaattca catatttgct tggcagagcc agctcatttc ctttttgtaa ttctccggca
      541 ctagccagca tccgtttacg accgttttct aactcaaaaa gactatattt aggtagttta
      601 atgattaagt cttttttaac ttccttatat cctttagctt ctaaaaagtc aatcggattt
      661 ttttcaaagg aacttctttc cataattgtg atccctagta actctttaac ggattttaac
      721 ttcttcgatt tccctttttc caccttagca accactagga ctgaataagc taccgttgga
      781 ctatcaaaac caccatattt t
//
