LOCUS       sequence-111987-        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLUK
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..1051
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 agcgcaaata agtctagttg cttcaggtgc tggtgctcgt ggaagctccg cagcagctta
       61 aggttgagca ttacgtatga tatgtgcatg cgattcggta atctccgaac agaaggaaga
      121 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
      181 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
      241 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc
      301 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt
      361 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact
      421 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa
      481 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg
      541 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat
      601 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga
      661 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc
      721 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt
      781 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt
      841 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag
      901 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt
      961 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc
     1021 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat
     1081 actaaactca caaattagag cttcaattta attatatcag ttattaccct atgcgg
//
