LOCUS       sequence-111990-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ubi-R as a Type 3a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK043
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..360
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     terminator      complement(500..534)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc atatgcagat tttcgttaaa actttaactg gtaaaacaat tactttagaa
      181 gttgagtcat ctgatactat cgataatgtt aaatctaaga tccaggataa ggaaggtatt
      241 cctccagatc aacaaagatt gattttcgca ggtaagcagt tggaggacgg taggactttg
      301 tctgattata acatacagaa agaatctact ttgcacttag tcttaagatt aaggggaggt
      361 agacatggtt ctggtgcctg gttgttacct gtatcattgg ttaaaagaaa gaccacattg
      421 gcaccaaata ctcaaactgc ctctccaaga gccttggcag actctttaat gcagagatca
      481 ggttcttgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      541 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
      601 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      661 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      721 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      781 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
      841 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      901 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
      961 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1021 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc at
//
