LOCUS       sequence-111994-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dCas9-VP64-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-dCas9-VP64-6xHis
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..47)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(66..83)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..233)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(264..329)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(336..356)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagt
       61 cattagtgat ggtggtggtg atgtaacata tcgagatcga aatcgtccag agcatcggag
      121 ccgagcatgt ccagatcaaa gtcatccaat gcgtcgcttc ccaacatatc caggtcaaag
      181 tcatcgaggg catcagaacc cagcatgtcg agatcgaaat cgtccagcgc gtcggcgcgc
      241 ccgcttccac cgcctcctgc agccttgtca tcgtcatcct tgtaatcgat gtcatgatct
      301 ttataatcac cgtcatggtc tttgtagtcg ctcgagactt tcctcttctt cttgggagaa
      361 ccgtcacctc ctagctgact caaatcaatg cgtgtttcat aaagaccagt gatggattga
      421 tggataagag tggcatctaa aacttctttt gtagacgtat atcgtttacg atcaattgtt
      481 gtatcaaaat atttaaaagc agcgggagct ccaagattcg tcaacgtaaa taaatgaata
      541 atattttctg cttgttcacg tattggtttg tctctatgtt tgttatatgc actaagaact
      601 ttatctaaat tggcatctgc taaaataaca cgcttagaaa attcactgat ttgctcaata
      661 atctcatcta aataatgctt atgctgctcc acaaacaatt gtttttgttc gttatcttct
      721 ggactaccct tcaacttttc ataatgacta gctaaatata aaaaattcac atatttgctt
      781 ggcagagcca gctcatttcc tttttgtaat tctccggcac tagccagcat ccgtttacga
      841 ccgttt
//
