LOCUS       sequence-111996-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of 6xHis-(-30)GFP in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-6xHis-(-30)GFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(798..815)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             828..850
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtacctc attattactt gtacagctcg tccattccat gatcaatgcc tgcagcggtc
      121 acgaattcta acagaaccat gtgatcgcgg tcttcgttcg gatctttgct cagggctgac
      181 tccgtgctca ggtagtgatc gtcaggtaac agtacaggcc catcaccaat cggcgtgttt
      241 tgttgataat gatccgctaa ttgaacgctg ccgtcctcca cgttatggcg aatttcaaat
      301 tctgccttga taccgttttc ctgtttatcg gccgtaatat acacgtcatg agaattaaag
      361 ttatattcca gcttatggcc taaaatgttg ccgtcttctt tgaagtcgat acctttcagt
      421 tcgatacggt ttactaatgt gtcgccttca aatttcactt ccgcacgcgt tttataggtg
      481 ccatcgtcct tgaagctgat tgtacgctct tgaacatatc cttcaggcat ggctgatttg
      541 aaaaaatcgt gttgatccat atgatctggg taatcactga agcactgaac gccataggtc
      601 agggtggtca ctaaggtggg ccacggaacg ggtaattcgc ctgttgtgca aataaacttc
      661 agggtcaatt caccctcggt agcgtcacct tcgccctcgc cgcgcacgct aaattcgtga
      721 ccgttcacgt cgccgtctaa ttccactaag atcggtacta caccgtcaaa cagctcttca
      781 cctttgctag cgccaccgtg atggtggtgg tgatgaccca tggtatatct ccttcttaaa
      841 gttaaacaaa
//
