LOCUS       sequence-111997-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 3b part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK046
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             136..843
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     terminator      complement(863..897)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc attctgtgtc caaaggagag gagttaatca aggaaaacat gagaatgaaa
      181 gttgtcatgg agggctccgt taatggtcac caattcaagt gtacagggga aggtgaaggt
      241 aatccttaca tgggtacaca aactatgaga attaaagtaa ttgaaggcgg accactacca
      301 tttgcatttg acattctggc aacgtcattc atgtacggat cacgaacttt catcaagtac
      361 cctaaaggta taccagactt tttcaagcaa tcttttccag agggttttac atgggaaagg
      421 gttacaagat acgaagatgg gggtgtcgtc acagttatgc aagatacttc attagaagat
      481 ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg
      541 atgcagaaaa agaccaaagg ttgggaacca aatactgaaa tgatgtaccc agctgatgga
      601 ggtttgagag gctacacaca catggcgctt aaagttgatg gtggaggtca tttgtcttgt
      661 agttttgtta ccacttatcg ttctaaaaag actgttggca atatcaaaat gccaggaata
      721 catgctgtag accacagact agaaagactc gaagagagcg ataacgaaat gttcgttgta
      781 cagagagagc atgccgtagc caaatttgct ggcttaggcg gtggtatgga tgaattgtat
      841 aagggatcct gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      901 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      961 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1021 tgccgacatg gaagccat
//
