LOCUS       sequence-112000-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes I-SceI recognition site as a Type 234 part to be used in the
            Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK049
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(170..204)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca aacgtaggga taacagggta atgctgtgag accagaccaa taaaaaacgc
      181 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
      241 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
      301 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
      361 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
      421 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
      481 tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg
      541 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
      601 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
      661 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
      721 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
      781 tatttttctt tacggtcttt aaaaagg
//
