LOCUS       sequence-112001-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK050
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             551..562
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctcc aacgatgtgc ttcagtatta cattttttgc cttcaacgcc ttgattgttc
      181 tatttttgct aataataaat ctatttcatc ggactaaaag tccattagtt gtaagcggat
      241 ttagctcagt tgggagagcg ccagactgaa gaaaaacttc ggtcaagtca tctggaggtc
      301 ctgtgttcga tccacagaat tcgcagatgg ccggcatggt cccagcctcc tcgctggcgc
      361 cggctgggca acaccttcgg gtggcgaatg ggacttgaga cggaaagtga aacgtgattt
      421 catgcgtcat tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt
      481 taatttatga atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta
      541 gctactagag aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt
      601 cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg
      661 cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg
      721 taaactgccg gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt
      781 tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg
      841 ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga
      901 a
//
