LOCUS       sequence-112003-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tSSA1 as a Type 4 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK052
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(387..421)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
       61 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      121 agtcggtctc aatcctaact cgaggccaat tggtgcggca attgataata acgaaaatgt
      181 cttttaatga tctgggtata atgaggaatt ttccgaacgt ttttacttta tatatatata
      241 tacatgtaac atatattcta tacgctatag agaaaggaaa tttttcaatt aaaaaaaaaa
      301 tagagaaaga gtttcacttc ttgattatcg ctaacactaa tggttgaagt actgctactt
      361 taattttatg ctgtgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
      421 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
      481 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
      541 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
      601 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
      661 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
      721 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
      781 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
      841 gatgaaaacg tttc
//
