LOCUS       sequence-112025-       11628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows the integration of myc Separase C2029A in the genome and 
            Tet-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Holland AJ, Taylor SS
  TITLE     Cyclin-B1-mediated inhibition of excess separase is required for 
            timely chromosome disjunction.
  JOURNAL   J Cell Sci. 2006 Aug 15;119(Pt 16):3325-36. Epub 2006 Jul 25.
  PUBMED    16868023
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-08-15"; volume: "119(Pt 16)"; pages: "3325-36. 
            Epub 2006 Jul 25"
COMMENT     Sequence Label: pcDNA5 FRT TO myc Separase C2029A
FEATURES             Location/Qualifiers
     source          1..11628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1154..1183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7580..7597)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7586..7810
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7943..7962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8094..8141
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8149..9171
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8149..9171
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9301..9422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9338..9357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9392..9411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9471..9487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9471..9487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9484..9506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9495..9511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9519..9525,9526..9543,9544..9549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9564..9585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9702..9719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9873..10461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9953..9972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10632..11423,11424..11492))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11255..11274
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11493..11597)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccc cggggaattc ggcagatccc gaggtccgac
     1021 agcgcccggc ccagatcccc acgcctgcca ggagcaagcc gagagccagc cggccggcgc
     1081 actccgactc cgagcagtct ctgtccttcg acccgagccc cgcgcccttt ccgggacccc
     1141 tgccccgcgg atggagcaga agctcatctc agaagaagac ctcgagagga gcttcaaaag
     1201 agtcaacttt gggactctgc taagcagcca gaaggaggct gaagagttgc tgcccgactt
     1261 gaaggagttc ctgtccaacc ctccagctgg ttttcccagc agccgatctg atgctgagag
     1321 gagacaagct tgtgatgcca tcctgagggc ttgcaaccag cagctgactg ctaagctagc
     1381 ttgccctagg catctgggga gcctgctgga gctggcagag ctggcctgtg atggctactt
     1441 agtgtctacc ccacagcgtc ctcccctcta cctggaacga attctctttg tcttactgcg
     1501 gaatgctgct gcacaaggaa gcccagaggc cacactccgc cttgctcagc ccctccatgc
     1561 ctgcttggtg cagtgctctc gcgaggctgc tccccaggac tatgaggccg tggctcgggg
     1621 cagcttttct ctgctttgga agggggcaga agccctgttg gaacggcgag ctgcatttgc
     1681 agctcggctg aaggccttga gcttcctagt actcttggag gatgaaagta ccccttgtga
     1741 ggttcctcac tttgcttctc caacagcctg tcgagcggta gctgcccatc agctatttga
     1801 tgccagtggc catggtctaa atgaagcaga tgctgatttc ctagatgacc tgctctccag
     1861 gcacgtgatc agagccttgg tgggtgagag agggagctct tctgggcttc tttctcccca
     1921 gagggccctc tgcctcttgg agctcacctt ggaacactgc cgtcgctttt gctggagccg
     1981 ccaccatgac aaagccatca gcgcagtgga gaaggctcac agttacctaa ggaacaccaa
     2041 tctagcccct agccttcagc tatgtcagct gggggttaag ctgctgcagg ttggggagga
     2101 aggacctcag gcagtggcca agcttctgat caaggcatca gctgtcctga gcaagagtat
     2161 ggaggcacca tcacccccac ttcgggcatt gtatgagagc tgccagttct tcctttcagg
     2221 cctggaacga ggcaccaaga ggcgctatag acttgatgcc attctgagcc tctttgcttt
     2281 tcttggaggg tactgctctc ttctgcagca gctgcgggat gatggtgtgt atgggggctc
     2341 ctccaagcaa cagcagtctt ttcttcagat gtactttcag ggacttcacc tctacactgt
     2401 ggtggtttat gactttgccc aaggctgtca gatagttgat ttggctgacc tgacccaact
     2461 agtggacagt tgtaaatcta ccgttgtctg gatgctggag gccttagagg gcctgtcggg
     2521 ccaagagctg acggaccaca tggggatgac cgcttcttac accagtaatt tggcctacag
     2581 cttctatagt cacaagctct atgccgaggc ctgtgccatc tctgagccgc tctgtcagca
     2641 cctgggtttg gtgaagccag gcacttatcc cgaggtgcct cctgagaagt tgcacaggtg
     2701 cttccggcta caagtagaga gtttgaagaa actgggtaaa caggcccagg gctgcaagat
     2761 ggtgattttg tggctggcag ccctgcaacc ctgtagccct gaacacatgg ctgagccagt
     2821 cactttctgg gttcgggtca agatggatgc ggccagggct ggagacaagg agctacagct
     2881 aaagactctg cgagacagcc tcagtggctg ggacccggag accctggccc tcctgctgag
     2941 ggaggagctg caggcctaca aggcggtgcg ggccgacact ggacaggaac gcttcaacat
     3001 catctgtgac ctcctggagc tgagccccga ggagacacca gccggggcct gggcacgagc
     3061 cacccacctg gtagaactgg ctcaggtgct ctgctaccac gactttacgc agcagaccaa
     3121 ctgctctgct ctggatgcta tccgggaagc cctgcagctt ctggactctg tgaggcctga
     3181 ggcccaggcc agagatcagc ttctggacga taaagcacag gccttgctgt ggctttacat
     3241 ctgtactctg gaagccaaaa tacaggaagg tatcgagcgg gatcggagag cccaggcccc
     3301 tggtaacttg gaggaatttg aagtcaatga cctgaactat gaagataaac tccaggaaga
     3361 tcgtttccta tacagtaaca ttgccttcaa cctggctgca gatgctgctc agtccaaatg
     3421 cctggaccaa gccctggccc tgtggaagga gctgcttaca aaggggcagg ccccagctgt
     3481 acggtgtctc cagcagacag cagcctcact gcagatccta gcagccctct accagctggt
     3541 ggcaaagccc atgcaggctc tggaggtcct cctgctgcta cggattgtct ctgagagact
     3601 gaaggaccac tcgaaggcag ctggctcctc ctgccacatc acccagctcc tcctgaccct
     3661 cggctgtccc agctatgccc agttacacct ggaagaggca gcatcgagcc tgaagcatct
     3721 cgatcagact actgacacat acctgctcct ttccctgacc tgtgatctgc ttcgaagtca
     3781 actctactgg actcaccaga aggtgaccaa gggtgtctct ctgctgctgt ctgtgcttcg
     3841 ggatcctgcc ctccagaagt cctccaaggc ttggtacttg ctgcgtgtcc aggtcctgca
     3901 gctggtggca gcttacctta gcctcccgtc aaacaacctc tcacactccc tgtgggagca
     3961 gctctgtgcc caaggctggc agacacctga gatagctctc atagactccc ataagctcct
     4021 ccgaagcatc atcctcctgc tgatgggcag tgacattctc tcaactcaga aagcagctgt
     4081 ggagacatcg tttttggact atggtgaaaa tctggtacaa aaatggcagg ttctttcaga
     4141 ggtgctgagc tgctcagaga agctggtctg ccacctgggc cgcctgggta gtgtgagtga
     4201 agccaaggcc ttttgcttgg aggccctaaa acttacaaca aagctgcaga taccacgcca
     4261 gtgtgccctg ttcctggtgc tgaagggcga gctggagctg gcccgcaatg acattgatct
     4321 ctgtcagtcg gacctgcagc aggttctgtt cttgcttgag tcttgcacag agtttggtgg
     4381 ggtgactcag cacctggact ctgtgaagaa ggtccacctg cagaagggga agcagcaggc
     4441 ccaggtcccc tgtcctccac agctcccaga ggaggagctc ttcctaagag gccctgctct
     4501 agagctggtg gccactgtgg ccaaggagcc tggccccata gcaccttcta caaactcctc
     4561 cccagtcttg aaaaccaagc cccagcccat acccaacttc ctgtcccatt cacccacctg
     4621 tgactgctcg ctctgcgcca gccctgtcct cacagcagtc tgtctgcgct gggtattggt
     4681 cacggcaggg gtgaggctgg ccatgggcca ccaagcccag ggtctggatc tgctgcaggt
     4741 cgtgctgaag ggctgtcctg aagccgctga gcgcctcacc caagctctcc aagcttccct
     4801 gaatcataaa acacccccct ccttggttcc aagcctcttg gatgagatct tggctcaagc
     4861 atacacactg ttggcactgg agggcctgaa ccagccatca aacgagagcc tgcagaaggt
     4921 tctacagtca gggctgaagt ttgtagcagc acggataccc cacctagagc cctggcgagc
     4981 cagcctgctc ttgatttggg ccctcacaaa actaggtggc ctcagctgct gtactaccca
     5041 actttttgca agctcctggg gctggcagcc accattaata aaaagtgtcc ctggctcaga
     5101 gccctctaag actcagggcc aaaaacgttc tggacgaggg cgccaaaagt tagcctctgc
     5161 tcccctgcgc ctcaataata cctctcagaa aggtctggaa ggtagaggac tgccctgcac
     5221 acctaaaccc ccagaccgga tcaggcaagc tggccctcat gtccccttca cggtgtttga
     5281 ggaagtctgc cctacagaga gcaagcctga agtaccccag gcccccaggg tacaacagag
     5341 agtccagacg cgcctcaagg tgaacttcag tgatgacagt gacttggaag accctgtctc
     5401 agctgaggcc tggctggcag aggagcctaa gagacggggc actgcttccc ggggccgggg
     5461 gcgagcaagg aagggcctga gcctaaagac ggatgccgtg gttgccccag gtagtgcccc
     5521 tgggaaccct ggcctgaatg gcaggagccg gagggccaag aaggtggcat caagacattg
     5581 tgaggagcgg cgtccccaga gggccagtga ccaggccagg cctggccctg agatcatgag
     5641 gaccatccct gaggaagaac tgactgacaa ctggagaaaa atgagctttg agatcctcag
     5701 gggctctgac ggggaagact cagcctcagg tgggaagact ccagctccgg gccctgaggc
     5761 agcttctgga gaatgggagc tgctgaggct ggattccagc aagaagaagc tgcccagccc
     5821 atgcccagac aaggagagtg acaaggacct tggtcctcgg ctccagctcc cctcagcccc
     5881 cgtagccact ggtctttcta ccctggactc catctgtgac tccctgagtg ttgctttccg
     5941 gggcattagt cactgtcctc ctagtgggct ctatgcccac ctctgccgct tcctggcctt
     6001 gtgcctgggc caccgggatc cttatgccac tgctttcctt gtcaccgagt ctgtctccat
     6061 cacctgtcgc caccagctgc tcacccacct ccacagacag ctcagcaagg cccagaagca
     6121 ccgaggatca cttgaaatag cagaccagct gcaggggctg agccttcagg agatgcctgg
     6181 agatgtcccc ctggcccgca tccagcgcct cttttccttc agggctttgg aatctggcca
     6241 cttcccccag cctgaaaagg agagtttcca ggagcgcctg gctctgatcc ccagtggggt
     6301 gactgtgtgt gtgttggccc tggccaccct ccagcccgga accgtgggca acaccctcct
     6361 gctgacccgg ctggaaaagg acagtccccc agtcagtgtg cagattccca ctggccagaa
     6421 caagcttcat ctgcgttcag tcctgaatga gtttgatgcc atccagaagg cacagaaaga
     6481 gaacagcagc tgtactgaca agcgagaatg gtggacaggg cggctggcac tggaccacag
     6541 gatggaggtt ctcatcgctt ccctagagaa gtctgtgctg ggctgctgga aggggctgct
     6601 gctgccgtcc agtgaggagc ccggccctgc ccaggaggcc tcccgcctac aggagctgct
     6661 acaggactgt ggctggaaat atcctgaccg cactctgctg aaaatcatgc tcagtggtgc
     6721 cggtgccctc acccctcagg acattcaggc cctggcctac gggctgtgcc caacccagcc
     6781 agagcgagcc caggagctcc tgaatgaggc agtaggacgt ctacagggcc tgacagtacc
     6841 aagcaatagc caccttgtct tggtcctaga caaggacttg cagaagctgc cgtgggaaag
     6901 catgcccagc ctccaagcac tgcctgtcac ccggctgccc tccttccgct tcctactcag
     6961 ctactccatc atcaaagagt atggggcctc gccagtgctg agtcaagggg tggatccacg
     7021 aagtaccttc tatgtcctga accctcacaa taacctgtca agcacagagg agcaatttcg
     7081 agccaatttc agcagtgaag ctggctggag aggagtggtt ggggaggtgc caagacctga
     7141 acaggtgcag gaagccctga caaagcatga tttgtatatc tatgcagggc atggggctgg
     7201 tgcccgcttc cttgatgggc aggctgtcct gcggctgagc tgtcgggcag tggccctgct
     7261 gtttggcgct agcagtgcgg ccctggctgt gcatggaaac ctggaggggg ctggcatcgt
     7321 gctcaagtac atcatggctg gttgcccctt gtttctgggt aatctctggg atgtgactga
     7381 ccgcgacatt gaccgctaca cggaagctct gctgcaaggc tggcttggag caggcccagg
     7441 ggcccccctt ctctactatg taaaccaggc ccgccaagct ccccgactca agtatcttat
     7501 tggggctgca cctatagcct atggcttgcc tgtctctctg cggtgagcgg ccgcgggccc
     7561 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7621 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7681 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7741 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7801 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7861 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7921 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7981 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8041 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc
     8101 ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa
     8161 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     8221 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     8281 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     8341 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
     8401 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     8461 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     8521 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     8581 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     8641 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     8701 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     8761 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     8821 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     8881 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     8941 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     9001 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     9061 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     9121 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta
     9181 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9241 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9301 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9361 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9421 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9481 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9541 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9601 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9661 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9841 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9901 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9961 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10021 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10081 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10141 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10201 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10261 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10321 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10381 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10441 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10501 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10561 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10621 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10681 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10741 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10801 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10861 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10921 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10981 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11041 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11101 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11161 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11221 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11281 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11341 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11401 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11461 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11521 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11581 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
