LOCUS       sequence-112030-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes six a1 operator sites upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 6x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..225)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 acaaagctgg agctccaccg cggtggcggc cggatcttcg agcgtcccaa aaccttctca
       61 agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa
      121 atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc
      181 tagacttcag gttgtctaac tccttccttt tcggttagag cggatcttag ctagccgcgg
      241 taccaagctt actcgagtta cttgtacaat tcgtccatac ccaaagtgat accagcagca
      301 gtacgaaatt ctaacaagac catgtgatct ctcttttcat ttgggtcctt agacaagaca
      361 gattgggtag acaagtaatg agaatctggt aataagactg gaccgtcacc aattggagtg
      421 ttttgttggt agtggtcagc taattggaca gaaccgtctt caacgttgtg tctgatttta
      481 aagttgacct taataccgtt cttttgctta acagccataa tgtaaatatt atgggagttg
      541 aaattgtatt ctaacttgtg acccaaaatg ttaccatctt ccttgaaatc aacacccttc
      601 aattcgattc tattgactaa agtatcacct tcgaacttga cttcggctct agttttgtaa
      661 gtaccatcgt ccttgaagaa gatagttctt tcttgaacgt aaccttctgg catggcgga
//
