LOCUS       sequence-112033-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes three a1 operator sites upstream of minimal pCYC1 promoter 
            driving yeast enhanced BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: 3x_a1gRNAOp_pCYC1m_yeBFP2
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(34..223)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 aaagctggag ctccaccgcg gtggcggccg gatcttcgag cgtcccaaaa ccttctcaag
       61 caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat
      121 ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa tagggaccta
      181 gacttcaggt tgtctaactc cttccttttc ggttagagcg gatcttagct agccgcggta
      241 ccaagcttac tcgagttact tgtacaattc gtccataccc aaagtgatac cagcagcagt
      301 acgaaattct aacaagacca tgtgatctct cttttcattt gggtccttag acaagacaga
      361 ttgggtagac aagtaatgag aatctggtaa taagactgga ccgtcaccaa ttggagtgtt
      421 ttgttggtag tggtcagcta attggacaga accgtcttca acgttgtgtc tgattttaaa
      481 gttgacctta ataccgttct tttgcttaac agccataatg taaatattat gggagttgaa
      541 attgtattct aacttgtgac ccaaaatgtt accatcttcc ttgaaatcaa cacccttcaa
      601 ttcgattcta ttgactaaag tatcaccttc gaacttgact tcggctctag ttttgtaagt
      661 accatcgtcc ttgaagaaga tagttctttc ttgaacgtaa ccttctggca tggcggattt
      721 aaagaaatca tgttgcttca tgtgatctgg gtaacgggcg aaacattgaa caccgtgaga
      781 caaagtg
//
