LOCUS       sequence-112038-       11326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BiFC CUC3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 11326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: JW777
FEATURES             Location/Qualifiers
     source          1..11326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(272..294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    380..520
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     435..454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(535..552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(706..1294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(786..805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1381..2175)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2600..2624
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    2702..2876
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2933..3730)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3044..3063)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3654..3673
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3761..3944)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3797..3976)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    4186..4207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4222..4227,4228..4245,4246..4252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4260..4276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4265..4287
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4284..4300
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4284..4300
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4811..5156
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5099..5119
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             6703..6726
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6736..6759
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      6780..7421
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(7429..7446)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7429..7445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7438..7460)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7648..7672
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8972..9601
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10035..11102
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      join(11168..11326,1..36)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
       61 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
      121 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
      181 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
      241 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      301 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      361 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      421 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      481 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
      541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1141 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     1381 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     1441 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     1501 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     1561 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     1621 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     1681 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     1741 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     1801 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     1861 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     1921 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     1981 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     2041 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     2101 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     2161 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     2221 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     2281 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     2341 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     2401 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     2461 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     2521 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     2581 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     2641 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     2701 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     2761 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     2821 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     2881 agcttgtcga tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga
     2941 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     3001 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     3061 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     3121 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat
     3181 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     3241 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     3301 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     3361 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     3421 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     3481 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     3541 cgtcctggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     3601 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     3661 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     3721 caatccccat ggtcgatcga cagatctgcg aaagctcgag tgcagattat ttggattgag
     3781 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     3841 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     3901 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     3961 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     4021 gggtcataac gtgactccct taattctccg ctcatgatcc caccatgttg gcaagctgct
     4081 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4141 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4201 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4261 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgggtc
     4321 cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt
     4381 acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc
     4441 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     4501 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     4561 ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat
     4621 caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg
     4681 gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     4741 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc
     4801 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
     4861 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
     4921 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
     4981 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     5041 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     5101 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg
     5161 ggacgagctc atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     5221 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     5281 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     5341 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     5401 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     5461 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     5521 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     5581 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgg gaggtggctc
     5641 atctggcgga ggtcagatct cgtacgcgtc ccggggcggt accatgatgc ttgcggtgga
     5701 agatgtgtta agcgaactcg ccggagaaga aaggaacgag agaggattgc cacctggctt
     5761 ccggtttcac ccgacggacg aagagctcat taccttctac ttagcttcca aaatcttcca
     5821 tggtggtctc tccggcattc acatttccga agttgatctc aaccgctgtg aaccttggga
     5881 gctaccagaa atggcgaaga tgggagagag agagtggtac ttttatagtc taagggacag
     5941 gaaatatccg acaggtttga ggactaacag agcaactact gctggatact ggaaagctac
     6001 cggcaaagat aaggaagtct tctccggcgg aggaggacag cttgttggga tgaagaagac
     6061 gttggtgttc tacaaaggta gggctccacg tggcctcaag actaagtggg tcatgcatga
     6121 gtatcgcctc gaaaacgacc attcacaccg ccacacgtgt aaggaggaat gggtgatttg
     6181 cagagtgttc aataaaacag gagacagaaa aaatgttgga ttaatccata accaaatcag
     6241 ctaccttcat aaccattcac tctcaacaac acatcatcat catcatgaag ccttaccttt
     6301 gcttatagaa ccttccaaca aaaccctaac caacttccca tcactactct acgatgatcc
     6361 acaccaaaac tacaataata acaacttcct tcatggatca tcaggccaca acatcgacga
     6421 gctcaaagcc ttaatcaacc ctgtcgtctc tcagctcaac ggtatcatct ttccttcagg
     6481 gaacaacaac aacgacgaag acgacttcga ctttaacctc ggcgtgaaaa cagagcagtc
     6541 ttcgaacggt aacgaaattg acgtacgaga ttacttggag aaccctctgt ttcaggaagc
     6601 gagttatggt ctgttgggtt tttcgtcttc tcctggacct cttcacatgc tactagattc
     6661 tccatgtcct ttaggattcc agctgtaggg atccaacaaa tggactacaa ggacgacgac
     6721 gacaaattaa ttaactggtc tcacccacag ttcgagaagc tcgagtagtc gacctgcaga
     6781 gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt
     6841 gcgcacacac cagaatccta ctgagttcga gtattatggc attgggaaac atgtttttct
     6901 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
     6961 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
     7021 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa atataagaga
     7081 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt
     7141 taatatgagg agtaaaacac ttgtagttgt accattatgc ttattcacta ggcaacaaat
     7201 atattttcag acctagaaaa gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt
     7261 ttagacattt atgaactttc ctttatgtaa ttttccagaa tccttgtcag attctaatca
     7321 ttgctttata attatagtta tactcatgga tttgtagttg agtatgaaaa tattttttaa
     7381 tgcattttat gacttgccaa ttgattgaca acatgcatca agcttggcac tggccgtcgt
     7441 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7501 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7561 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     7621 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     7681 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
     7741 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     7801 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     7861 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     7921 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     7981 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     8041 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     8101 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     8161 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     8221 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     8281 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     8341 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     8401 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     8461 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     8521 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     8581 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     8641 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     8701 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     8761 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     8821 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     8881 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     8941 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     9001 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     9061 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     9121 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     9181 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     9241 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     9301 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     9361 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     9421 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     9481 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     9541 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     9601 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     9661 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     9721 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     9781 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     9841 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     9901 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     9961 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
    10021 ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
    10081 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
    10141 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
    10201 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
    10261 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
    10321 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
    10381 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
    10441 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
    10501 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
    10561 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
    10621 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
    10681 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
    10741 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
    10801 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
    10861 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
    10921 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
    10981 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
    11041 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
    11101 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
    11161 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
    11221 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
    11281 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaact
//
