LOCUS       sequence-112042-       11108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BiFC empty vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Gou JY, Felippes FF, Liu CJ, Weigel D, Wang JW
  TITLE     Negative regulation of anthocyanin biosynthesis in Arabidopsis by a 
            miR156-targeted SPL transcription factor.
  JOURNAL   Plant Cell. 2011 Apr;23(4):1512-22. doi: 10.1105/tpc.111.084525. 
            Epub 2011 Apr 12.
  PUBMED    21487097
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.084525"; journalName: "Plant Cell"; date: 
            "2011-04"; volume: "23"; issue: "4"; pages: "1512-22"
COMMENT     Sequence Label: JW771
FEATURES             Location/Qualifiers
     source          1..11108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(272..294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    380..520
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     435..454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(535..552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(706..1294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(786..805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1381..2175)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2600..2624
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    2702..2876
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2933..3730)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3044..3063)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3654..3673
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3761..3944)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3797..3976)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    4186..4207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4222..4227,4228..4245,4246..4252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4260..4276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4265..4287
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4284..4300
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4284..4300
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4810..5155
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5098..5118
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5192..5215
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5225..5248
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(5371..5389)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      6562..7203
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(7211..7228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7211..7227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7220..7242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7430..7454
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8754..9383
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9817..10884
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      join(10950..11108,1..36)
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
       61 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
      121 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
      181 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
      241 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      301 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      361 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      421 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      481 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
      541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1141 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1321 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     1381 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     1441 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     1501 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     1561 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     1621 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     1681 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     1741 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     1801 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     1861 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     1921 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     1981 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     2041 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     2101 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     2161 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     2221 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     2281 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     2341 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     2401 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     2461 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     2521 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     2581 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     2641 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg
     2701 ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt
     2761 attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc
     2821 ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg
     2881 agcttgtcga tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga
     2941 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     3001 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     3061 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     3121 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat
     3181 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     3241 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     3301 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     3361 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     3421 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     3481 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     3541 cgtcctggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     3601 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     3661 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     3721 caatccccat ggtcgatcga cagatctgcg aaagctcgag tgcagattat ttggattgag
     3781 agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt
     3841 tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa
     3901 tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc
     3961 ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg
     4021 gggtcataac gtgactccct taattctccg ctcatgatcc caccatgttg gcaagctgct
     4081 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4141 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     4201 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     4261 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcggtcc
     4321 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     4381 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     4441 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     4501 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     4561 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4621 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4681 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4741 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4801 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4861 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4921 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4981 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     5041 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     5101 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     5161 gacgagctcg gtacccggga tccaacaaat ggactacaag gacgacgacg acaaattaat
     5221 taactggtct cacccacagt tcgagaagct cgagtagtcg accagatctc gtacgcgtcc
     5281 cggggcggtg gctcatctgg cggaggtgaa gacgccaaaa acataaagaa aggcccggcg
     5341 ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga
     5401 tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact
     5461 tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg
     5521 aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg
     5581 ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt
     5641 gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg
     5701 ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg
     5761 gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta
     5821 cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt
     5881 gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat
     5941 agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt
     6001 ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca
     6061 ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg
     6121 tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc
     6181 tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt
     6241 gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat
     6301 ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca
     6361 cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag
     6421 gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg
     6481 agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt
     6541 gacaaggatg gatgactgca gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca
     6601 agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttc gagtattatg
     6661 gcattgggaa acatgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6721 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6781 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6841 ttgaatttga aaatataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6901 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat
     6961 gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa
     7021 tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag
     7081 aatccttgtc agattctaat cattgcttta taattatagt tatactcatg gatttgtagt
     7141 tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat
     7201 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7261 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7321 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga
     7381 gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
     7441 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc
     7501 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     7561 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     7621 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     7681 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     7741 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     7801 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     7861 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     7921 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     7981 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     8041 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     8101 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     8161 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     8221 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     8281 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     8341 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     8401 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     8461 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     8521 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     8581 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     8641 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     8701 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     8761 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     8821 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     8881 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     8941 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     9001 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     9061 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     9121 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     9181 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     9241 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     9301 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     9361 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     9421 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     9481 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     9541 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     9601 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     9661 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     9721 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     9781 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
     9841 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     9901 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     9961 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    10021 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    10081 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    10141 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    10201 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    10261 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    10321 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    10381 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    10441 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    10501 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    10561 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    10621 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    10681 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    10741 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    10801 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    10861 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    10921 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
    10981 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    11041 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    11101 ttaaaact
//
