LOCUS       sequence-112048-        9247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TCP4 expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Rubio-Somoza I, Zhou CM, Confraria A, Martinho C, von Born P, 
            Baena-Gonzalez E, Wang JW, Weigel D
  TITLE     Temporal control of leaf complexity by miRNA-regulated licensing of 
            protein complexes.
  JOURNAL   Curr Biol. 2014 Nov 17;24(22):2714-9. doi: 
            10.1016/j.cub.2014.09.058. Epub 2014 Nov 6.
  PUBMED    25448000
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2014.09.058"; journalName: "Curr Biol"; date: 
            "2014-11-17- 17"; volume: "24"; issue: "22"; pages: "2714-9"
COMMENT     Sequence Label: JW630
FEATURES             Location/Qualifiers
     source          1..9247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1476
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1522..1542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1558..1899
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1905..1924
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1905..1923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1930..1939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1942..1968
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      3675..3862
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3730..3754)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(3979..5073)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5074..5479)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5521..5537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5521..5537)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5534..5556)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5545..5561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5569..5575,5576..5593,5594..5599))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5614..5635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5690..5723
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5801..5834
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5903..5920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6074..6662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6154..6173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6833..7624,7625..7693))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7456..7475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7694..7798)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7866..7884
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7922..7944)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8044..8063
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8080..9244
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa
     1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc
     1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt
     1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt
     1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat
     1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg
     1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta
     1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat
     1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg
     1981 gaggccagtg aattccaccc gggtgggcat cgatacggga tccatatgtc tgacgaccaa
     2041 ttccatcacc cgccgcctcc ttcttcaatg aggcaccgtt ctacgtcgga tgcggcggac
     2101 ggcggctgcg gcgagattgt tgaggtgcaa ggtggtcaca ttgttcggtc taccggaaga
     2161 aaagaccgcc acagcaaagt ctgcacggct aaagggccac gtgaccggcg cgtgagactc
     2221 tctgctcaca cggcgattca gttttacgat gttcaagaca ggcttggttt cgaccgacct
     2281 agcaaagccg ttgattggct tatcaaaaag gctaagactt ccattgacga gctcgctgag
     2341 cttcctccct ggaatcccgc cgatgcaatt cgcctagccg ctgctaacgc taaacccaga
     2401 agaaccaccg ccaaaaccca aatctctccg tctccgccac cgccgcaaca gcaacaacaa
     2461 caacaacagc ttcagttcgg tgttggcttc aacggaggag gagcagagca tccgagtaac
     2521 aacgagtcga gttttctccc gccgtcaatg gattcagatt cgatagctga cactataaag
     2581 tcgttttttc cggtgattgg ctcttcaacg gaggctcctt cgaatcataa ccttatgcac
     2641 aactatcatc atcagcatcc gccggatttg ctttctcgaa ctaatagcca aaaccaagat
     2701 ctccgtctct cgctgcaatc gttcccggat ggtccaccgt cgcttctgca ccaccaacat
     2761 caccaccaca cctctgcttc cgcctccgag cctactctgt tctacggaca gagcaatccg
     2821 ttagggtttg acacatcgag ttgggagcag cagtcgtcgg aattcggaag gattcagaga
     2881 ctagtggctt ggaacagcgg cggtggcggc ggagcaaccg atacaggaaa cggaggaggg
     2941 tttctgttcg ctcctcctac tccttcaacg acgtcgtttc agccagttct tggccaaagc
     3001 caacagcttt attctcagag gggtcccctt cagtccagtt acagtcccat gatccgtgct
     3061 tggtttgatc ctcaccatca tcaccaatcc atctccaccg acgatctcaa ccaccaccat
     3121 caccttcctc caccggttca ccaatcagca atccccggaa tcggattcgc ctcaggtgaa
     3181 ttctcttcgg gttttcgcat accagcacgg tttcagggcc aagaagagga gcagcacgac
     3241 ggtctcactc acaagccgtc ctctgcttcc tctatttctc gccattaact cgagctgcag
     3301 atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc gtgcaccatc
     3361 tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac tcctctaagt
     3421 ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta gaattaatgc
     3481 ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct tattcagaag
     3541 ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta
     3601 ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg
     3661 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     3721 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat
     3781 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc
     3841 ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac atattccatt
     3901 ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca
     3961 taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc
     4021 gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac
     4081 ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa
     4141 catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg
     4201 agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc
     4261 caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt catcggagat
     4321 gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac
     4381 taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt
     4441 cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt
     4501 atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct
     4561 tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc
     4621 atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa
     4681 gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc
     4741 aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc
     4801 ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac
     4861 ggcatcaacc ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcgtc
     4921 gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc
     4981 agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc
     5041 tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata
     5101 tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac
     5161 ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa
     5221 gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct
     5281 ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat
     5341 taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc
     5401 gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata
     5461 cctgagtatt cccacagttg gggatctcga ctctagctag aggatcaatt cgtaatcatg
     5521 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5581 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5641 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga taacttcgta
     5701 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctagc
     5761 ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg
     5821 ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5881 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5941 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6001 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6061 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6121 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6181 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6241 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6301 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6361 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6421 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6481 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6541 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6601 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6661 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6721 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6781 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6841 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6901 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6961 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7021 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7081 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7141 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7201 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7261 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7321 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7381 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7441 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7501 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7561 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7621 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7681 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7741 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7801 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7861 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7921 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7981 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     8041 atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg
     8101 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
     8161 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
     8221 aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt
     8281 cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc
     8341 accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa
     8401 agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt
     8461 cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt
     8521 actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg
     8581 aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa
     8641 ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat
     8701 ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct
     8761 tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata
     8821 gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag
     8881 actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa
     8941 tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa
     9001 aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
     9061 aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt
     9121 ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
     9181 tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt
     9241 cgttgct
//
