LOCUS       sequence-112055-         502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 502)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MDC1 in pENTR4 (330-5)
FEATURES             Location/Qualifiers
     source          1..502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      224..267
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      399..426
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     434..457
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagcatg gatctcgggg
      181 acgtctaact actaagcgag agtagggaac tgccaggcat caaataaaac gaaaggccca
      241 gtcttccgac tgagcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      301 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc
      361 aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg
      421 cctttttgcg tttctacaaa ctcttcctgt tagttagtta cttaagctcg ggccccaaat
      481 aatgatttta ttttgactga ta
//
