LOCUS       sequence-112059-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Hung YP, Albeck JG, Tantama M, Yellen G
  TITLE     Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
            Fluorescent Biosensor.
  JOURNAL   Cell Metab. 2011 Oct 5;14(4):545-54.
  PUBMED    21982714
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT     Sequence Label: GW1-Peredox-mCitrine
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    15..149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(39..58)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     93..112
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(274..525)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(316..337)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     665..684
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     924..945
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgagctcgg tacggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
       61 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      121 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      181 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      241 tggctgatta tgatcaatga attcgtcgag ttacttgtac agttcatcca tacccagagt
      301 gatcccagca gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      361 tttgctcagt ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc
      421 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      481 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      541 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      601 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      661 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      721 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      781 catcaggccg tagccgaagg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      841 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      901 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      961 ggtgaacagc tcctcg
//
