LOCUS       sequence-112073-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP 
            and Puro resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, 
            Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
  TITLE     Isolation of human iPS cells using EOS lentiviral vectors to select 
            for pluripotency.
  JOURNAL   Nat Methods. 2009 May . 6(5):370-6.
  PUBMED    19404254
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 May . 6(5):370-6."
COMMENT     Sequence Label: PL-SIN-EOS-C(3+)-EiP
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgctcac catggtggct ttaccaacag taccggaatg ccaagcttac ttagatcgca
       61 gatcttgttg cggccgccag cagctcgcaa cgtgaacggt tcgactgaga aggccgctcg
      121 agctttaaga gaggaatcta gacggggcgg aagaaagaat gtagccaaga cagttactct
      181 gatcaaggct caaattttat agttgcgaca ctagttatga aggaaggggg aggggacccg
      241 attcccgccg aataatctct ggtccagtag aaaggtgcac gggtgtggct ccgcaggttc
      301 cagcagtggg cgtggcagaa cgaatgagca ggaagctcca cccctgagca agcaggtttc
      361 aggctggggg aggggagaat tcactagtga ttaagctcga gctaggacga gagggacccc
      421 tccccaacca tcttctctgc ccccagggca gagctatcat gcacctttgt tatgcatctg
      481 ccgtctgccc agaactctca acctccctgg ggggaggggc agggtcgggc tacaacctcc
      541 ccacacccga attcactagt gattaagctc gagctaggac gagagggacc cctccccaac
      601 catcttctct gcccc
//
