LOCUS       sequence-112105-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + 
            PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2073
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(355..382)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(474..560)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     713..730
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(816..836)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgattaaag cttgggcctt cctgatacat aaaaaaacgc ctgctcttat tacggagcag
       61 gcgttaaaac aggtctgtat gacaacaagt gggtgcttca ctcaacgttg tgtccatggt
      121 gtctgatgag gcataagcga catctgtcag tggacgataa gcaccgtaaa cggctctgcg
      181 tcattccgga gtttatgagg cactaaggcg aacataagag atggaatgag catctacccc
      241 gggattataa ccgccaagca taaaaatgtc aacaacgacg gtgctcgagg atcctgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaaagag tttgtagaaa cgcaaaaagg
      361 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
      421 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
      481 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      541 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
      601 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
      661 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
      721 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccaagctagc ttttatttgt
      781 agagctcatc catgccatgt gtaatcccag cagctgttac aaactcaaga aggaccatgt
      841 ggtctctctt ttcgttggga tctttcgaaa gggca
//
