LOCUS       sequence-112127-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tribolium basal heat shock promoter driving Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Gilles AF, Schinko JB, Averof M
  TITLE     Efficient CRISPR-mediated gene targeting and transgene replacement 
            in the beetle Tribolium castaneum.
  JOURNAL   Development. 2015 Jul 9. pii: dev.125054.
  PUBMED    26160901
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Jul 9. pii: dev.125054."
COMMENT     Sequence Label: p(bhsp68-Cas9)
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             441..461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      639..648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             645..4748
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             645..4748
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4755..4775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5222..5239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5222..5238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5451..5906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5538..5557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5748..5769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5923..5942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6042..6064
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6102..6120)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6188..6292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6293..6361,6362..7153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6511..6530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7324..7912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7813..7832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8066..8083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8200..8221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8236..8241,8242..8259,8260..8266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8274..8290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8279..8301
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8298..8314
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8298..8314
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattggcgc gcctaggccg gccgaattcg aatggccatg ggacgtcgac ctgaggtaat
       61 tataacccgg gccctatata tggatccaat tgcaatgatc atcatgacag atctgcgcgc
      121 gatcgatatc cgtttcatat ataagcgcgg tctcgcggcg cgttgtcagt gaatttgaat
      181 caagcgaagc gaaagcaaca aagctaagca agcgactcaa agcaaatttt caaagcgact
      241 ttaacaaagt tgagtgaatt atttactagt gaattcaaag tcgtcgataa ataagtttcc
      301 catttttatt ccccagccag gaagttagtt tcaatagttt tgtaatttca acgaaactca
      361 tttgatttcg tactaatttt ccacatctct attttctgcc cgcagaataa tccaaaatgc
      421 agatcgggga tcccacccca cccaagaaga agcgcaaggt ggaggacgat cccgtcgttt
      481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      601 tgcggtcgac tctagaggat ccccgggatc caccggtcgc caccatggat aagaaatact
      661 caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
      721 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
      781 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
      841 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
      901 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
      961 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
     1021 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
     1081 ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
     1141 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
     1201 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
     1261 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
     1321 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
     1381 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
     1441 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
     1501 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
     1561 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
     1621 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
     1681 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
     1741 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
     1801 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
     1861 acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac
     1921 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
     1981 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
     2041 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
     2101 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
     2161 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
     2221 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
     2281 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
     2341 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
     2401 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
     2461 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
     2521 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
     2581 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
     2641 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
     2701 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
     2761 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
     2821 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
     2881 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
     2941 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
     3001 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
     3061 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
     3121 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt
     3181 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
     3241 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
     3301 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
     3361 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
     3421 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
     3481 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
     3541 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
     3601 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
     3661 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
     3721 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
     3781 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
     3841 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
     3901 tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct
     3961 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
     4021 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
     4081 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
     4141 tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
     4201 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
     4261 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
     4321 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
     4381 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
     4441 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
     4501 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
     4561 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
     4621 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
     4681 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
     4741 gaggtgacgg ttctcccaag aagaagagga aagtctcgag cggtggagct gcaggatgag
     4801 cggccgcctg cagctggcgc catcgatacg cgtacgtcgc gaccgcggac atgtacagag
     4861 ctcgagatga tcggtttgtg ttcctactta gttaattgtg atagtttatt tttgtaaata
     4921 atagtacgta aagttacttt agttataata aagtagatag tgactgcatt aagtttaaag
     4981 actgattttt gtagattatt taaaaatttt taataaatta tttaatttaa agataaaaag
     5041 ttgtttcatt ttaaaataat aaaaaactta ccgcattagt cgtggccttg gcagtattaa
     5101 gcgcaaacgg gctctccttc gtatgcccat caggaatata gaaaaaaaca actggctgct
     5161 cactagtggc cacgtgggcc gtgcacctta agcttggcgc gcctaggccg gccagcttgg
     5221 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5281 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     5341 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     5401 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     5461 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5521 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5581 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5641 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     5701 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5761 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     5821 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5881 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     5941 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6001 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     6061 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     6121 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6301 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6361 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6421 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6481 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6541 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6601 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6661 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6721 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6781 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6841 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6901 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6961 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7021 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7081 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7141 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7201 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7441 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7981 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8041 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8101 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8161 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8221 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8281 cggataacaa tttcacacag gaaacagcta tgaccatgat tac
//
