LOCUS       sequence-112131-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + 
            PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2073
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    258..276
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    283..301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(322..340)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        408..512
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(731..750)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttcgcatgc tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt
       61 cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt
      121 tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccat gtgaccgggt
      181 tagccggcca gaatcacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg
      241 gcacacggtc gacagtctct ctatcactga tagggatgtc aatctctatc actgataggg
      301 actcgaggtg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      361 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      721 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      901 acactgcggc caacttactt ctgacaccga tcgga
//
