LOCUS       sequence-112132-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL836
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        212..316
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(535..554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatgctag ctgggaccag gagggacagt actttaactt tcatgttatt aaccctctgt
       61 tatatgcctt tatttgcttt tttatgccac tgcatactga ttaaccctca ttaatcagtc
      121 ggcaagtcga cagtatcttg ttatccgctc acaatgtcaa tgttatccgc tcacatttat
      181 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      541 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      841 gcgtgacacc acgatgcctg tagcaatggc aacaacgt
//
