LOCUS       sequence-112134-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + 
            PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1954
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(257..284)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(376..462)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcgattaaag cttgtggata aattgagaac gaaagatcaa aaaaaaagcc agcacccggc
       61 tggctaagta atactggaag caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg
      121 tcttcctgat cgccccgggg aatccaccat aaccgccaag cataaaaatg taaacaacga
      181 cggtgctcga ggatcctgag cggatacata tttgaatgta tttagaaaaa taaacaaaag
      241 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct
      301 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcn caacgttcaa
      361 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      421 acgaaaggcc cagtctttcn actgagcctt tcgttttatt tgatgcctgg cagttcccta
      481 ctctcgcatg gggagacccc acactacca
//
