LOCUS       sequence-112140-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + 
            PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL518
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             116..127
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    282..298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(391..418)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(510..596)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     749..766
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(852..872)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
       61 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatc gtacgtttct
      121 cctctttaat aagcttctgg ataaggatta tccaattcta aaaaaaaagc ccggacgact
      181 gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc ccaacagaaa
      241 ataaaatctg tgacaataaa ggcatataac ccgggagtat cttgttatcc gctcacaatg
      301 tcaatgttat ccgctcacat ttatggatcc tgagcggata catatttgaa tgtatttaga
      361 aaaataaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      421 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      481 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      541 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
      601 ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt
      661 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt
      721 ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc
      781 tcatccgcca aaacagccaa gctagctttt atttgtagag ctcatccatg ccatgtgtaa
      841 tcccagcagc tgttacaaac tcaagaagga ccatgtgg
//
