LOCUS       sequence-112143-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + 
            ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1017
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(70..164)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttctcggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact
       61 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      121 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgtaaa
      181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catag
//
