LOCUS       sequence-112145-         468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::ipex::mCherry::T1 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL718
FEATURES             Location/Qualifiers
     source          1..468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             109..120
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    413..431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    438..456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca cccttgaagc gcatgaactc
       61 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atcgtacgtt tctcctcttt
      121 aataagcttc cggttagaga tggatatcgt tgttaataac tctaattaat atgccaattg
      181 tttactaaaa attattaaaa atggggcgtt gagacgcccc caaaaataaa gggtaatata
      241 taacagaagg tttatatagt tagaagcaag gttgtgcttc taaaggaagt ggcttgaggg
      301 agccacttat atgttgggga ggcaacgcct cccgcaacat atctttttcg taatcagatt
      361 agaactggta aaccagacct acagcaacga tgtcatcagt gccccgggag tatctctatc
      421 actgataggg atgtcaatct ctatcactga tagggaggat cctgagcg
//
