LOCUS       sequence-112149-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 4a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK058
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             135..848
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(872..906)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctca atcctctaaa ggtgaagaat tattcactgg tgttgtccca attttggttg
      181 aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg
      241 ctacttacgg taaattgacc ttaaaattga tttgtactac tggtaaattg ccagttccat
      301 ggccaacctt agtcactact ttaggttatg gtttgcaatg ttttgctaga tacccagatc
      361 atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt caagaaagaa
      421 ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag tttgaaggtg
      481 ataccttagt taatagaatc gaattaaaag gtattgattt taaagaaggt ggtaacattt
      541 taggtcacaa attggaatac aactataact ctcacaatgt ttacatcact gctgacaaac
      601 aaaagaatgg tatcaaagct aacttcaaaa ttagacacaa cattgaagat ggtggtgttc
      661 aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc ttgttaccag
      721 acaaccatta cttatcctat caatctgcct tatccaaaga tccaaacgaa aagagagatc
      781 acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatg gatgaattgt
      841 acaaataact cgagtggctg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
      901 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
      961 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     1021 taagccatcc g
//
