LOCUS       sequence-112151-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL391
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    458..476
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    483..501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(568..595)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(687..773)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
       61 gatgatggcc atgttatcct cctcgccctt gctcaccatc gtacgtttct cctctttaat
      121 aagcttattc ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat
      181 gatggtagtt actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag
      241 acgggacaac agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca
      301 ggatcacgaa ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat
      361 aaatagaaac tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt
      421 gccccttagc catttttttt cctccttacc gggagtatct ctatcactga tagggatgtc
      481 aatctctatc actgataggg aggatcctga gcggatacat atttgaatgt atttagaaaa
      541 ataaacaaaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      601 atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      661 gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      721 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
      781 gcagttccct actctcgcat
//
