LOCUS       sequence-112154-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + 
            PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL417
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     626..643
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(729..749)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacgattaaa gcttctggat aaggattatc caattctaaa aaaaaagccc ggacgactgt
       61 tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc aacagaaaat
      121 aaaatctgtg acaataaagg catataaccc gggagtatct ctatcactga tagggatgtc
      181 aatctctatc actgataggg aggatcctga gcggatacat atttgaatgt atttagaaaa
      241 ataaacaaaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac
      541 ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg
      601 ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca
      661 tccgccaaaa cagccaagct agcttttatt tgtagagctc atccatgcca tgtgtaatcc
      721 cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg
      781 aaagggc
//
