LOCUS       sequence-112156-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + 
            PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL2117
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(254..281)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(373..459)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     612..629
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(715..735)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 attaaagctt gtggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg
       61 ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct
      121 tcctgatcgc cccggggaat ccaccataac cgccaagcat aaaaatgtaa acaacgacgg
      181 tgctcgagga tcctgagcgg atacatattt gaatgtattt agaaaaataa acaaaagagt
      241 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc
      301 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc
      361 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
      421 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc
      481 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
      541 tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc
      601 ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacagc
      661 caagctagct tttatttgta gagctcatcc atgccatgtg taatcccagc agctgttaca
      721 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt
      781 gtggacaggt aatggttgtc tggtaaaagg acagggcc
//
