LOCUS       sequence-112159-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + 
            PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2
            terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1954
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(280..298)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        366..470
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(689..708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatgctcg cggtaccttt aatcaggttg ttcaggttag tgacataagt ctgatggtgc
       61 ttgccgtagt gatactcgat ggtttccgca gaaatgtgcg gtgccagagc atctttagca
      121 tatggtagtg caggtaattc gaatgacatt gctactctcc tttattatta atttgcatac
      181 gtacaatagc cttattgtgc gtatggtcga cagtctctct atcactgata gggatgtcaa
      241 tctctatcac tgatagggac tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt
      301 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      361 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      481 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      541 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      601 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      661 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      721 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      781 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      841 ccataaccat gagtgataac actgcggcca acttacttct ga
//
