LOCUS       sequence-112160-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tENO1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK061
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(377..411)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
       61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      121 gtcggtctcc tggcagcttt tgattaagcc ttctagtcca aaaaacacgt ttttttgtca
      181 tttatttcat tttcttagaa tagtttagtt tattcatttt atagtcacga atgttttatg
      241 attctatata gggttgcaaa caagcatttt tcattttatg ttaaaacaat ttcaggttta
      301 ccttttattc tgcttgtggt gacgcgtgta tccgcccgct cttttggtca cccatgtatg
      361 ctgtgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
      421 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
      481 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      541 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      601 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      661 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
      721 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      781 aa
//
