LOCUS       sequence-112161-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + 
            PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL518
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(199..217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        285..389
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(608..627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcatgctagc tgggaccagg agggacagta ctttaacttt catgttatta accctctgtt
       61 atatgccttt atttgctttt ttatgccact gcatactgat taaccctcat taatcagtcg
      121 gcaagtcgac agtctctcta tcactgatag ggatgtcaat ctctatcact gatagggact
      181 cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaaagcat
      721 cttacggatg gcatgacagt aagagaatta tgcagtggct gccataaccc atgagtgata
//
