LOCUS       sequence-112164-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + 
            ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sagawa S, Shin JE, Hussein R, Lim HN
  TITLE     Paradoxical suppression of small RNA activity at high Hfq 
            concentrations due to random-order binding.
  JOURNAL   Nucleic Acids Res. 2015 Aug 10. pii: gkv777.
  PUBMED    26261213
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Aug 10. pii: gkv777."
COMMENT     Sequence Label: pHL1017
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        163..267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..830
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(486..505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatgctaa ccatgactaa ctcctttttt atcatctctg gaacacacac aatccgtgtg
       61 caggtccctg ccagagtcga cgaatccacc ataaccgcca agcataaaaa tgtaaacaac
      121 acggtgctcg agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      841 ttaactggcg
//
