LOCUS       sequence-112171-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLK
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        334..741
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 agcgcaaata agtctagttg cttcaggtgc tggtgctcgt ggaagctccg cagcagctta
       61 aggttgagca ttacgtatga tatgtgcgcg ccatcgagga gaacttctag tatatctaca
      121 tacctaatat tattgcctta ttaaaaatgg aatcccaaca attacatcaa aatccacatt
      181 ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt
      241 ataggataat tatactctat ttctcaacaa gtaattggtt gtttggccga gcggtctaag
      301 gcgcctgatt caagaaatat cttgaccgca gttaactgtg ggaatactca ggtatcgtaa
      361 gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata acgagaacac
      421 acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga
      481 ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga
      541 gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca
      601 caatacttga agttgacaat attatttaag gacctattgt tttttccaat aggtggttag
      661 caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata tatatatata
      721 tttcaaggat ataccattct aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac
      781 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt
      841 cgttcca
//
